Table 1.
Ranking of candidate reference genes across eggs of haplochromine cichlid species using three different softwares
| BestKeeper | geNorm | NormFinder | |||||
|---|---|---|---|---|---|---|---|
| Ranking | SD | Ranking | r | Ranking | M | Ranking | SV |
| NF | 0.930 | NF | 0.805 | NF | 1.287 | NF | 0.602 |
| mid1ip1 | 0.954 | atf7ip | 0.781 | atf7ip | 1.344 | atf7ip | 0.615 |
| ccna1 | 0.969 | ccnb2 | 0.761 | mid1ip1 | 1.405 | ccnb2 | 0.688 |
| atf7ip | 1.112 | mid1ip1 | 0.679 | actb1 | 1.511 | actb1 | 0.688 |
| actb1 | 1.286 | actb1 | 0.678 | tatdn2 | 1.519 | xpo6 | 0.766 |
| tbp | 1.331 | mylipa | 0.635 | xpo6 | 1.522 | mid1ip1 | 0.785 |
| ccnb2 | 1.500 | rps18 | 0.617 | ccnb2 | 1.522 | tatdn2 | 0.814 |
| rps18 | 1.566 | tbp | 0.592 | gapdh | 1.528 | mylipa | 0.815 |
| mylipa | 1.575 | ccna1 | 0.485 | ccna1 | 1.592 | ccna1 | 0.900 |
| xpo6 | 1.587 | xpo6 | – | tbp | 1.634 | gapdh | 0.903 |
| tatdn2 | 1.630 | tatdn2 | – | mylipa | 1.653 | tbp | 0.911 |
| gapdh | 1.655 | gapdh | – | rps18 | 1.752 | rps18 | 1.028 |
| elf1a | 1.692 | elf1a | – | dvr1 | 1.771 | dvr1 | 1.042 |
| rps11 | 1.991 | rps11 | – | rps11 | 1.943 | elf1a | 1.050 |
| dvr1 | 2.045 | dvr1 | – | elf1a | 2.044 | rps11 | 1.134 |
SD standard deviation, r Pearson product-moment correlation coefficient, SV stability value, M M value of stability, NF normalization factor, indicating geometric mean for Cq values of atf7ip and mid1ip1 genes