Table 5.
Phenolic compounds from Mašnjača, Krvavica and Leccino VOOs hydrofilic fraction.
| No. | Compounds | RT | LOD | LOQ | Mašnjača | Krvavica | Leccino | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (mg/kg) | (mg/kg) | (mg/kg) | (mg/kg) | (mg/kg) | |||||||||
| Min | Max | Mean ± SD | Min | Max | Mean ± SD | Min | Max | Mean ± SD | |||||
| 1. | Hydroxytyrosol a | 10.9 | 0.34 | 1.02 | 3.9 | 5.0 | 4.4 ± 0.8 | 14.9 | 21.9 | 18.4 ± 5.0 | 4.3 | 6.8 | 5.5 ± 1.8 |
| 2. | Tyrosol a | 13.4 | 0.40 | 1.21 | 3.9 | 4.9 | 4.4 ± 0.7 | 18.9 | 24.7 | 21.8 ± 4.1 | 3.0 | 4.8 | 3.9 ± 1.3 |
| 3. | Vanillic acid a | 15.1 | 0.04 | 0.11 | nd | 0.1 | 0.4 | 0.3 ± 0.2 | nd | ||||
| 4. | p-Coumaric acid a | 19.2 | 0.23 | 0.70 | nd | 1.2 | 1.3 | 1.3 ± 0.1 | nd | ||||
| 5. | 3,4-DHPEA-EDA b | 22.4 | 0.94 | 2.86 | 60.2 | 64.2 | 62.2 ± 2.8 | 20.5 | 29.2 | 24.8 ± 6.2 | 146.5 | 159.7 | 153.1 ± 9.3 |
| 6. | Pinoresinol a | 25.2 | 0.16 | 0.47 | 0.5 | 2.1 | 1.3 ± 1.1 | 2.1 | 2.4 | 2.3 ± 0.2 | 2.7 | 3.2 | 3.0 ± 0.4 |
| 7. | Luteolin a | 25.7 | 0.46 | 1.40 | 4.8 | 5.1 | 4.9 ± 0.2 | 8.6 | 8.7 | 8.6 ± 0.0 | 5.0 | 6.2 | 5.6 ± 0.9 |
| 8. | p-HPEA-EDA b | 26.3 | 0.94 | 2.86 | 45.2 | 46.0 | 45.6 ± 0.6 | 40.2 | 45.6 | 42.9 ± 3.9 | 124.1 | 141.7 | 132.9 ± 12.5 |
| 9. | Apigenin a | 28.2 | 0.36 | 1.10 | 2.1 | 2.5 | 2.3 ± 0.3 | 2.7 | 2.9 | 2.8 ± 0.2 | 2.1 | 2.6 | 2.3 ± 0.3 |
| 10. | 3,4-HPEA-EA b | 28.5 | 0.94 | 2.86 | 60.9 | 85.4 | 73.2 ± 17.3 | 73.0 | 95.5 | 84.2 ± 15.9 | 110.6 | 138.2 | 124.4 ± 19.5 |
rt = retention time; Min. = minimal value; Max. = maximal value; mean. = average value; LOD = limit of detection; LOQ = limit of quantification; SD. = standard deviation; nd = not detected (<LOD); a Identification based on RT and UV-Vis spectra of pure compounds; b Tentative identification by UV-Vis spectra and comparison of retention times with the literature data, and compounds dosed using the oleuropein calibration curve; 3,4-DHPEA-EDA, dialdehydic form of decarboxymethyl elenolic acid linked to hydroxytyrosol (decarboxymethyl oleuropein aglycon); p-HPEA-EDA, dialdehydic form of decarboxymethyl elenolic acid linked to tyrosol (decarboxymethyl ligstroside aglycon); 3,4-DHPEA-EA, oleuropein aglycon.