Table 2.

Sequences of unmodified and modified siRNAs targeting the Renilla luciferase mRNA and scrambled (SCR) siRNA; Tm (±0.5 °C) and Median Inhibition Concentration (IC₅₀) values (mean ± SD). : L-threoninol-thymine monomer; : thymidine monomer with phosphorothioate linkage; T: thymidine.

siRNA	ON	Sequence	Tm [°C]	IC50 [pM]
AE1	SS1	TTAAAAAGAGGAAGAAGUCUA-5′	67.8	9.8 ± 0.2
	AS1	5′-UUUUUCUCCUUCUUCAGAUTT
AE2	SS1	TTAAAAAGAGGAAGAAGUCUA-5′	N.D.	6.3 ± 0.5
	AS2	5′-UUUUUCUCCUUCUUCAGAU
AE3	SS2	AAAAAGAGGAAGAAGUCUA-5′	N.D.	14.3 ± 0.3
	AS1	5′-UUUUUCUCCUUCUUCAGAUTT
AE4	SS2	AAAAAGAGGAAGAAGUCUA-5′	67.4	7.2 ± 0.4
	AS2	5′-UUUUUCUCCUUCUUCAGAU
AES2	SS1	TTAAAAAGAGGAAGAAGUCUA-5′	N.D.	6.5 ± 0.2
	ASP	5′- UUUUUCUCCUUCUUCAGAU
AES3	SSP	AAAAAGAGGAAGAAGUCUA-5′	N.D.	10.5 ± 0.4
	AS1	5′-UUUUUCUCCUUCUUCAGAUTT
AES4	SSP	AAAAAGAGGAAGAAGUCUA-5′	67.8	8.3 ± 0.3
	ASP	5′-UUUUUCUCCUUCUUCAGAU
SCR	SS3	TTAGGAAAGAAAGAAAGCUAU-5′	N.D.	Not active
	AS3	5′-UCCUUUCUUUCUUUCGAUATT