Figure 3.

Inhibition of hTNF-α binding to adalimumab by branched anti-TNF-α peptide in SPR. A, adalimumab was captured via Protein A on a CM5 sensor chip as described in the Experimental Section. hTNF-α was flowed in the presence of different concentrations (1, 10 and 25 µM) of branched anti-TNF peptide. B, Curve of binding inhibition of hTNF-α binding to adalimumab derived by experiment in A. y axis indicates the percentage of binding between adalimumab and hTNF-α; x axis represents the concentration of tetra-branched anti-TNF peptide. The IC₅₀ is indicated.