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On-line HPLC-ABTS+• radical quenching 2D chromatograms of E. hirta crude ethanol extract. A 10 μL of E. hirta extract was analyzed by gradient reverse-phase-HPLC with a DAD at 254 nm (positive trace) prior to reaction with ABTS+• radical and the analysis of antioxidant potential at 734 nm (negative trace).
