Table 2.
Intra-day and inter-day precision and accuracy for the determination of four components.
| Nominal conc.(ng/mL) | Intra-day | Inter-day | ||||
|---|---|---|---|---|---|---|
| Observed conc. (ng/mL) | Precision, RSD (%) | Accuracy, Bias (%) | Observed conc. (ng/mL) | Precision, RSD (%) | Accuracy, Bias (%) | |
| Gentiopicroside | ||||||
| 10 | 9.82 ± 0.98 | 10.0 | −1.8 | 10.1 ± 1.45 | 14.4 | 0.9 |
| 25 | 23.5 ± 2.68 | 11.4 | −6.0 | 25.7 ± 2.11 | 8.2 | 2.9 |
| 50 | 49.9 ± 4.29 | 8.6 | −0.3 | 49.2 ± 1.26 | 2.6 | −1.6 |
| 100 | 98.1 ± 2.52 | 2.6 | −1.9 | 99.6 ± 2.91 | 2.9 | −0.4 |
| 250 | 259 ± 6.67 | 2.6 | 3.5 | 251 ± 5.72 | 2.3 | 0.3 |
| 500 | 494 ± 5.09 | 1.0 | −1.1 | 500 ± 2.82 | 0.6 | −0.1 |
| Geniposide | ||||||
| 10 | 9.37 ± 0.88 | 9.4 | −6.3 | 9.77 ± 1.16 | 11.9 | −2.3 |
| 25 | 25.4 ± 1.19 | 4.7 | 1.5 | 26.0 ± 1.96 | 7.5 | 3.9 |
| 50 | 49.5 ± 1.01 | 2.0 | −0.9 | 49.8 ± 3.06 | 6.1 | −0.4 |
| 100 | 98.2 ± 4.04 | 4.1 | −1.8 | 100 ± 3.02 | 3.0 | 0.1 |
| 250 | 256 ± 5.66 | 2.2 | 2.3 | 249 ± 3.50 | 1.4 | −0.4 |
| 500 | 497 ± 7.04 | 1.4 | −0.5 | 500 ± 1.48 | 0.3 | 0.1 |
| Baicalin | ||||||
| 10 | 11.0 ± 1.01 | 9.1 | 10.1 | 10.2 ± 1.51 | 14.8 | 2.1 |
| 25 | 24.9 ± 1.65 | 6.6 | −0.3 | 25.8 ± 2.70 | 10.5 | 3.3 |
| 50 | 50.9 ± 1.92 | 3.8 | 1.9 | 47.3 ± 1.12 | 2.4 | −5.4 |
| 100 | 98.4 ± 4.51 | 4.6 | −1.6 | 99.3 ± 3.04 | 3.1 | −0.7 |
| 250 | 245 ± 8.61 | 3.5 | −1.9 | 250 ± 4.71 | 1.9 | −0.1 |
| 500 | 491 ± 19.6 | 4.0 | −1.7 | 505 ± 9.20 | 1.8 | 1.0 |
| Swertiamarin | ||||||
| 5 | 5.35 ± 0.33 | 6.2 | 7.0 | 4.74 ± 0.25 | 5.3 | −5.1 |
| 10 | 9.91 ± 0.66 | 6.7 | −0.9 | 10.1 ± 0.53 | 5.2 | 1.0 |
| 25 | 24.8 ± 0.37 | 1.5 | −0.9 | 24.9 ± 0.75 | 3.0 | −0.4 |
| 50 | 50.0 ± 1.06 | 2.1 | −0.1 | 49.8 ± 1.16 | 2.3 | −0.4 |
| 100 | 99.6 ± 1.28 | 1.3 | −0.4 | 101 ± 1.35 | 1.3 | 0.9 |
| 250 | 251 ± 2.20 | 0.9 | 0.5 | 253 ± 7.21 | 2.9 | 1.1 |
Data are expressed as mean ± S.D. (n = 6).