Figure 2.

(A) Reversed-phase HPLC separation pattern on a C₁₈ column of the active fraction FI from Figure 1D. The gradient conditions were: 0–1.5 min, 2% methanol; 1.5–10 min, linear from 2% to 30% methanol. Elutions were performed at a flow rate of 0.5 mL/min using a UV detector at 220 nm. (B) Identification of molecular mass and amino acid sequences of P1, MS/MS experiments were performed on ESI Q-TOF MS. (C) Identification of molecular mass and amino acid sequences of P2, MS/MS experiments were performed on ESI Q-TOF MS. P1 and P2 were sequenced via de novo sequencing using Biolynx software.