Table 2.
In vitro growth inhibition of selected tumor cell lines by some benzimidazole-4,7-diones.
| Compounds | IC50 [μM] | Differential Cytotoxicity O/H | ||
|---|---|---|---|---|
| Normoxia (O) | Hypoxia (H) | |||
| 1a | A549 | 30.2 ± 1.2 | 36.1 ± 1.2 | 0.83 |
| WM 115 | 26.4 ± 1.6 | 32.5 ± 1.2 | 0.81 | |
| 1b | A549 | 100.0 ± 1.8 | 47.4 ± 1.1 | 2.13 |
| WM 115 | 101.0 ± 1.2 | 45.8 ± 1.8 | 2.20 | |
| 1c | A549 | 80.9 ± 1.9 | 51.2 ± 1.5 | 1.58 |
| WM 115 | 77.9 ± 1.3 | 48.5 ± 1.3 | 1.60 | |
| 1d | A549 | 479.5 ± 3.6 | 232.4 ± 1.4 | 2.06 |
| WM 115 | 399.5 ± 2.2 | 229.2 ± 0.4 | 1.74 | |
| 2a | A549 | 115.7 ± 1.9 | 35.0 ± 1.6 | 3.28 |
| WM 115 | 105.0 ± 0.7 | 33.0 ± 1.9 | 3.18 | |
| 2b | A549 | 500.6 ± 1.2 | 116.0 ± 0.8 | 4.31 |
| WM 115 | 490.6 ± 1.9 | 115.0 ± 1.2 | 4.26 | |
| 2c | A549 | 79.5 ± 1.9 | 44.0 ± 2.5 | 1.80 |
| WM 115 | 65.5 ± 1.3 | 40.0 ± 2.0 | 1.63 | |
| 2d | A549 | 252 ± 2.5 | 96.8 ± 1.9 | 2.59 |
| WM 115 | 189 ± 1.5 | 95.2 ± 2.1 | 1.98 | |
| T | A549 | 166.2 ± 1.6 | 36.0 ± 1.2 | 4.61 |
| WM 115 | 156.2 ± 1.6 | 34.0 ± 1.2 | 4.59 | |
WST-1 assay was used to determine the inhibition of the cell growth. After a 48 h incubation with the tested compounds. IC50 values (concentration of the tested compounds causing. 50% inhibition of the cell growth compared to control cells) were calculated and expressed as the mean ± SD, n = 3.