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. 2014 Dec 5;19(12):20350–20373. doi: 10.3390/molecules191220350

Figure 5.

Figure 5

(A) Electrophoretic profile (R-SDS-PAGE) of total globulins from Lupinus albus seeds (lane 1) versus purified β-conglutin (lane 2). Incubation of erythrocyte membranes with β-conglutin, followed by R-SDS-PAGE and Coomassie Blue R staining revealed that at least three polypeptides bound to the membranes (lane 3). Lanes 4 and 5: initial membrane and final membrane, obtained before incubation and after elution with 0.4 M galactose, respectively; (B) An identical gel was blotted onto a nitrocellulose membrane and incubated with anti-β-conglutin antibodies; (C,D) Vicia faba electrophoretic study (R-SDS-PAGE) and immunodetection with anti-V. faba vicilin polypeptides, respectively, analogous to (A) and (B). Molecular masses of standards (MM) are indicated in kDa. Black dots indicate polypeptides bound to erythrocyte membranes.