Table 2.
PCR conditions
| Exon | Pre-denaturation | Denaturation | Annealing | Extension | Final extension | Cycle |
|---|---|---|---|---|---|---|
| Exon1 | 95 °C/5 min | 95 °C/1 min | 63 °C/30 sec | 72 °C/1 min | 72 °C/5 min | 35 |
| Exon2 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/1 min | 72 °C/5 min | 35 |
| Exon3 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/30 sec | 72 °C/5 min | 35 |
| Exon4 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/30 sec | 72 °C/5 min | 35 |
| Exon5 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/30 sec | 72 °C/5 min | 35 |
| Exon6 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/30 sec | 72 °C/5 min | 35 |
| Exon7 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/1 min | 72 °C/5 min | 35 |
| Exon8 | 95 °C/5 min | 95 °C/30 sec | 63 °C/30 sec | 72 °C/30 sec | 72 °C/5 min | 35 |