Figure 2.

Heat stress (HS) and camel whey protein (CWP) impact on the expression of Bcl-2 family members and on the expression of Survivin. (A) Western blotting analysis were performed on white blood cell lysates of control, HS and CWP-HS animal groups using anti-Bcl-2, anti-Survivin, anti-Bax, anti-Bim, and anti-cytochrome C antibodies. The protein bands from one representative experiment are shown, and the expression of all indicated proteins was normalized to total β–actin protein levels. Data from five mice per group are expressed as the mean expression values ± SEM for (B) Bcl-2; (C) Survivin; (D) Bax; (E) Bim; and (F) cytochrome C. The data were analyzed by ANOVA with Tukey’s post hoc test. Differences were considered statistically significant at *P<0.05 for HS vs. control; +P<0.05 for CWP-HS vs. control; and #P<0.05 CWP- HS vs. HS