Figure 2.

PIG3 promotes non‐small cell lung cancer (NSCLC) cell migration. PIG3 knockdown (A) and overexpression (D) were verified in A549 and H1299 cells by western blot. The cell migration of A549 cells transfected with PIG3‐special siRNA (siPIG3) #1, #2 or non‐targeting control siRNA (siNC) (B) and H1299 cells transfected with PIG3 constructs (PIG3) or empty vector (pCMV) (E) was determined as described in the Materials and Methods. Representative images of the migrated cells are shown. Scale bar = 100 μm. Histogram of relative migration distance of transfected A549 cells (C) and H1299 cells (F) determined by measuring the distance between the scratch. Data were presented as mean ± SD from 3 independent experiments. Compared with the corresponding control, *P < .05; ***P < .001. G, Representative images of single cell migration of A549 cells transfected with siPIG3 #1 or siNC. Scale bar = 10 μm. H, Migration distance of single A549 cell was measured using Image J software. Data were presented as mean ± SD from 3 independent experiments, with at least 30 cells measured in each experiment. ***P < .001 compared with siNC group