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. 2018 Oct 26;109(12):3783–3793. doi: 10.1111/cas.13818

Figure 6.

Figure 6

FAK inhibition reverses metastatic characteristics of non‐small cell lung cancer (NSCLC) cells induced by PIG3 overexpression. A, H1299 cells transfected with PIG3 constructs were treated with the FAK inhibitor (FAKi) PF‐573228 at different concentrations (0, 5, 10 μmol/L) for 24 h. Inhibition efficiency of PF‐573228 on FAK phosphorylation was examined by western blot. B, The wound‐healing assay of transfected H1299 cells treated with different concentrations of PF‐573228. Representative images of the migrated cells are shown. Scale bar = 100 μm. C, Histogram of relative migration distance of transfected H1299 cells determined by measuring the distance between the scratch. Data were presented as mean ± SD from 3 independent experiments. *P < .05; **P < .01; ***P < .001 compared with control cells. D, Transwell assay of transfected H1299 cells treated with PF‐573228 at different concentrations. Representative images of the invading cells are shown (200 ×  magnification). E, Histogram of invading cells per field of transfected H1299 cells determined by counting the invading cells in 5 random fields. Data were presented as mean ± SD from 3 independent experiments (*P < .05; **P < .01; ***P < .001). F, PIG3 silencing sensitizes A549 cells to PF‐573228. Twenty‐four hours after transfection with PIG3 and control siRNA, A549 cells were exposed to PF‐573228 at different concentrations (0, 1.25, 2.5, 5, 10, 20 μmol/L). Cell proliferation was determined by CCK8 assay 72 h post‐treatment. Data were presented as mean ± SD from 3 independent experiments (**P < .01; ***P < .001)