Table 2.
Association between regulatory factors of Mieap expression and subtypes
| Case | Mieap methylated | NIX methylated | BNIP3 methylated | p53 mutation | Mieap expression | Subtype |
|---|---|---|---|---|---|---|
| 1 | + | − | − | − | − | Luminal B |
| 4 | + | − | − | − | − | Luminal B |
| 15 | + | − | − | − | − | Luminal B |
| 28 | + | − | − | − | − | Luminal B |
| 39 | + | − | − | − | − | Luminal B |
| 55 | + | − | − | − | − | Luminal B |
| 10 | − | − | − | Deletion in exon 4 | − | Triple negative |
| 32 | − | − | − | Deletion in exon 4 | − | Triple negative |
| 35 | − | − | − | Deletion in exon 7 | − | Triple negative |
| 42 | − | − | − | TGC→TGG(176C→W) | + | Triple negative |
| 52 | − | − | − | CGT→TGT (273R→C) | − | Luminal B |
| 56 | − | − | − | GAC→CAC (281D→H) | NE | HER2 |
| 13 | − | − | − | − | + | Luminal A |
| 33 | − | − | − | − | + | Triple negative |
| 40 | − | − | − | − | + | Luminal B |
| 44 | − | − | − | − | + | Luminal A |
| 49 | − | − | − | − | + | Luminal B |
| 50 | − | − | − | − | + | Luminal B |
| 2 | − | − | − | − | − | Luminal A |
| 6 | − | − | − | − | − | HER2 |
| 11 | − | − | − | − | − | Luminal/HER2 |
| 12 | − | − | − | − | NE | Luminal/HER2 |
| 16 | − | − | − | − | − | Luminal A |
| 17 | − | − | − | − | − | Luminal A |
| 18 | − | − | − | − | − | Luminal B |
| 19 | − | − | − | − | − | Luminal A |
| 20 | − | − | − | − | − | Luminal A |
| 22 | − | − | − | − | − | Luminal A |
| 23 | − | − | − | − | − | Luminal B |
| 24 | − | − | − | − | − | Luminal A |
| 25 | − | − | − | − | − | Luminal A |
| 26 | − | − | − | − | − | Luminal B |
| 27 | − | − | − | − | − | Luminal B |
| 29 | − | − | − | − | NE | Luminal B |
| 30 | − | − | − | − | − | Luminal A |
| 31 | − | − | − | − | − | Luminal A |
| 34 | − | − | − | − | NE | Luminal A |
| 37 | − | − | − | − | − | Triple negative |
| 38 | − | − | − | − | − | Luminal A |
| 41 | − | − | − | − | − | Luminal B |
| 43 | − | − | − | − | − | Triple negative |
| 46 | − | − | − | − | − | Luminal A |
| 47 | − | − | − | − | − | Luminal A |
| 48 | − | − | − | − | NE | Luminal A |
| 53 | − | − | − | − | − | Luminal B |
| 54 | − | − | − | − | − | Luminal A |
| 6/46 (13%) | 0/46 (0%) | 0/46 (0%) | 6/46 (13%) | 7/41 (17%) |
NE, not examined.
Results of MSP for Mieap/NIX/BNIP3 promoters and p53‐mutation search (exons 4‐8) are summarized. Methylation of the Mieap promoter was positive in 6/46 (13%) cases, whereas NIX/BNIP3 promoters were negative. Genetic alterations in p53 were detected in 6/46 (13%) cases. Finally, inactivation of the p53/Mieap/NIX/BNIP3‐regulated mitochondrial quality control (MQC) pathway was observed in 12/46 (26.1%) cases. These cases were aggressive phenotypes such as Luminal B, triple negative and HER2‐enriched. Among them, ten cases of 11 subjected to IHC lost Mieap expression.