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. 2018 Oct 30;109(12):3840–3852. doi: 10.1111/cas.13803

Figure 3.

Figure 3

Cell proliferation and migration assays of LNCaP cells in which tripartite motif 36 (TRIM36) is overexpressed or knocked down. A, mRNA levels of TRIM36 were determined by quantitative RT‐PCR in TRIM36‐transfected LNCaP cells (n = 3, bar = SD; **P < .001, Student's t test). B, Protein levels of TRIM36 were analyzed in TRIM36‐transfected LNCaP cells by western blotting. C, MTS assay of TRIM36‐transfected LNCaP cells (bar = SD; *P < .05, Student's t test). D, Representative pictures of migration assay. TRIM36‐transfected and control‐treated LNCaP cells are shown. Scale bar = 10 μm. E, Quantification of migrated cells of TRIM36‐transfected and control‐treated LNCaP cells (bar = SD; **P < .001, Student's t test). F, mRNA levels of TRIM36 were determined by quantitative RT‐PCR in siTRIM36‐transfected LNCaP cells (n = 3, bar = SD; **P < .001, Student's t test). G, Protein levels of TRIM36 were analyzed in siTRIM36‐transfected LNCaP cells by western blotting. H, MTS assay of siTRIM36‐transfected LNCaP cells (bar = SD; *P < .05, Student's t test). I, Representative pictures of migration assay. siTRIM36‐transfected and control‐treated LNCaP cells are shown. Scale bar = 10 μm. J, Quantification of migrated cells of siTRIM36‐transfected and control‐treated LNCaP cells (bar = SD; *P < .05, Student's t test). IB, immunoblot