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. 2018 Nov 5;109(12):3726–3736. doi: 10.1111/cas.13811

Figure 3.

Figure 3

γ‐Interferon (IFN‐γ), but not type I IFN, upregulates indoleamine 2,3‐dioxygenase 1 in T cells (T‐IDO1) through the JAK2‐STAT1 signaling pathway. A‐C, Correlation studies were carried out for IFNG,CD8A, and IDO1 mRNA expression in hepatocellular carcinoma patients’ tumor tissues; GAPDH was used as an internal control (n = 20). D,E, Western blot detection of IDO1 expression in Hep3B and Huh7 cell lines in the presence or absence of 20 ng/mL IFN‐γ, 1000 IU/mL (D) or 20 ng/mL (E) type I IFN for 48 hours. F,G, Western blot detection of IDO1 expression in Hep3B cell lines (F) and Huh7 cell lines (G) in the presence or absence of 20 ng/mL IFN‐γ for 24 hours. This effect could be abrogated by the JAK2 inhibitor (JAK2i) or STAT1 inhibitor (STAT1i) but not by the JAK1/3 inhibitor (JAK1/3i). Total protein (40 μg) was loaded in each lane