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. 2015 Feb 11;20(2):2903–2921. doi: 10.3390/molecules20022903

Table 6.

Independent variables and their levels for Box-Behnken design of hydrolysis reaction.

Independent Variables Symbol Variable Levels
−1 0 1
pH X1 4.5 5.0 5.5
Temperature (°C) X2 40 50 60
Enzyme quantity (mg) X3 25 50 75