Figure 3.

Representative profiles of direct inhibition of human plasmin by sulfated molecules. The inhibition of plasmin was measured spectrophotometrically through a chromogenic substrate hydrolysis assay at pH 7.4 and 37 °C. Solid lines represent sigmoidal fits to the data to obtain IC₅₀, HS, Y_M, and Y_O using Equation (1), as described in the Experimental Procedures.