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. 2015 Jan 5;20(1):608–624. doi: 10.3390/molecules20010608

Figure 4.

Figure 4

Michaelis-Menten kinetics of chromogenic substrate hydrolysis by human plasmin in the presence of sulfated flavonoid-quinazolinone heterodimer (32) (A) and flavonoid homodimer (52) (B). The initial rate of hydrolysis at various substrate concentrations was measured spectrophotometrically in pH 7.4 buffer at 37 °C. Solid lines represent nonlinear regressional fits to the data by the standard Michaelis-Menten equation to yield KM and VMAX.