Table 1.
Summarization of the GlcDH purification from A. globiformis C224.
| Step | Protein (mg) | Enzyme Activity (Units) | Specific Activity (Units/mg) | Recovery (%) | Purification (Fold) |
|---|---|---|---|---|---|
| Membrane fraction | 9758.1 ± 121.3 | 4730.6 ± 96.5 | 0.5 | 100.0 | 1.0 |
| Triton X-114 phase separation | 6004.5 ± 96.1 | 4396.4 ± 79.8 | 0.7 | 92.9 | 1.5 |
| Acetone precipitation | 1103.7 ± 30.6 | 1524.9 ± 34.2 | 1.4 | 32.2 | 2.9 |
| PEG precipitation | 408.8 ± 17.4 | 846.3 ± 12.3 | 2.1 | 17.9 | 4.3 |
| Ethanol precipitation | 33.6 ± 1.2 | 268.2 ± 6.4 | 7.9 | 5. 7 | 16.6 |
| Hydroxylapatite fraction | 1.8 ± 0.1 | 159.4 ± 2.1 | 88.1 | 3.4 | 183.5 |