Figure 1.

Effects of the BMP inhibitor dorsomorphin on bone mineralization. Alizarin red staining of 5 dpf larvae treated at 2, 3 or 4 dpf during 24 h with 100 µM dorsomorphin. Control embryos were treated with DMSO. Embryos treated at 2 dpf (B lateral view and D ventral view) show severe reduction of all mineralized bone pieces compared to the controls (A lateral view and C ventral view). The treatments starting at 3 dpf (F in ventral view) and 4 dpf (H in ventral view) lead to a reduction of bone mineralization, but to a lesser extent than at 2 dpf compared to the controls embryos (respectively E and G in ventral view). c: cleithrum; en: entopterygoid; o: operculum; p: parasphenoid. Scale bar: 200 µM.