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(A–D) Effects of BMP inhibitor treatment at 2–3 dpf on cartilage formation. Alcian blue staining of 5 dpf larvae previously treated at 2 dpf during 24 h with 100 µM dorsomorphin (B) or 20 µM K02288 (D). Control embryos (A,C) were treated with DMSO; (E–H) In situ hybridization using the sox9a probe. No difference in cartilage formation was observed between control (E,G) and treated (F,H). cb 1–4: ceratobranchial ray 1 to 4; ch: ceratohyal; mk: Meckel’s cartilage. Scale bar: 200 µM.
