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. 2015 Apr 24;20(5):7586–7601. doi: 10.3390/molecules20057586

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© 2015 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effects caused by expression of the dominant negative Bmpr on bone formation. (A–C) Alizarin red staining of 5 dpf larvae after heat-shock treatment at 2 or 3 dpf. The extent of GFP expression 2 h after treatment indicates the transgenic status of the larva; GFP(−) being non-transgenic, GFP(++) transgenic, GFP(+++) being homozygous transgenic or larvae highly expressing the transgene (dnBmpr-GFP); (D–F) DAF-FM DA staining for nitric oxide detection in 5 dpf larvae (note that GFP fluorescence has disappeared at this time); (G–I) Red fluorescence allowing detection of mCherry-expressing osteoblasts c: cleithrum; en: entopterygoid; o: operculum; p: parasphenoid. Scale bar: 200 µM. (J,K) Statistical analysis of replicate experiments investigating the effect of dnBmpr expression on nitric oxide (J) or mCherry (K) detection. * indicates p-value < 0.05.