Table 2.

Intra-day and inter-day precision and accuracy of the six active components of HGW.

Nominal Concentration (ng/mL)	Intra-Day			Inter-Day
	Observed Concentration (ng/mL)	Accuracy (%)	Precision (%)	Observed Concentration (ng/mL)	Accuracy (%)	Precision (%)
Astragaloside I
25	25.21 ± 1.49	0.86	5.90	23.83 ± 0.85	−4.68	3.55
50	47.68 ± 3.93	−4.64	8.24	48.78 ± 1.73	−2.44	3.56
250	259.48 ± 5.70	3.79	2.20	237.78 ± 5.93	−4.89	2.49
500	488.38 ±15.04	−2.32	3.08	512.51 ± 13.76	2.50	2.69
1000	987.19 ± 9.88	−1.28	1.00	983.27 ± 22.24	−1.67	2.26
Astragaloside IV
10	9.23 ± 0.46	−7.72	4.98	9.82 ± 1.34	−1.84	13.61
50	23.64 ± 1.44	5.43	6.08	49.21 ± 3.29	−1.58	6.68
250	50.21 ± 1.14	0.41	2.26	250.03 ± 7.69	0.01	3.08
500	246.48 ± 8.80	−1.41	3.57	515.21 ± 5.45	3.04	1.06
1000	517.22 ± 6.27	3.44	1.21	997.18 ± 0.68	−0.28	0.07
Formononetin
25	22.81 ± 1.33	−8.75	5.82	22.08 ± 0.47	−11.67	2.12
50	46.01 ± 1.93	−7.98	4.19	46.77 ± 2.34	−6.46	5.01
250	253.44 ± 10.43	1.38	4.11	254.42 ± 5.85	1.77	2.30
500	506.04 ± 10.04	1.21	1.98	509.04 ± 15.79	1.81	3.10
1000	995.55 ± 2.29	−0.44	0.23	994.74 ± 6.45	−0.53	0.65
Cinnamic acid
25	27.69 ± 1.95	10.74	7.05	26.32 ± 1.53	5.29	5.83
50	53.33 ± 1.62	6.65	3.04	49.83 ± 3.40	−0.33	6.82
250	243.41 ± 5.31	−2.64	2.18	236.01 ± 11.10	−5.60	4.70
500	497.55 ± 10.83	−0.49	2.18	487.97 ± 23.74	−2.41	4.87
1000	1001.63 ± 5.97	0.16	0.60	997.47 ± 8.11	−0.25	0.81
Paeoniflorin
25	24.99 ± 1.55	−0.04	6.21	24.50 ± 3.31	−2.01	13.50
50	49.44 ± 3.38	−1.13	6.83	51.09 ± 4.00	2.17	7.84
250	257.68 ± 16.41	3.07	6.37	245.60 ± 10.78	−1.76	4.39
500	501.05 ± 7.91	0.21	1.58	505.76 ± 1.82	1.15	0.36
1000	993.47 ± 9.92	−0.65	1.00	998.49 ± 0.90	−0.15	0.09
Gingerol
10	9.58 ± 0.84	−4.25	8.82	9.26 ± 0.47	7.38	5.10
50	49.13 ± 1.79	−1.74	3.65	50.31 ± 2.26	0.61	4.49
250	251.93 ± 2.66	0.77	1.05	251.84 ± 7.15	0.73	2.84
500	500.08 ± 7.34	0.02	1.47	497.17 ± 8.01	−0.57	1.61
1000	999.65 ± 3.05	−0.04	0.31	1000.92 ± 2.27	0.09	0.23

Each value is expressed as the mean ± S.E.M (n = 3).