Figure 5.

Effect of hispidin on the translocation of NF-κB in palmitate-treated C2C12 myotubes. C2C12 myotubes were cultured with indicated concentration of hispidin for 8 h, and then exposed to 1 mM palmitate for 16 h. (A) The expression levels of fractional NF-κB were assessed using western blot analysis. (B) Quantitative analysis of the band density of fractional NF-κB. The equal loading of the total proteins in each sample was confirmed by the expression of β-actin. Data represent the mean ± standard error of three independent experiments. * p < 0.05, as compared with only BSA group, ^# p < 0.05, as compared with PA group. NF-κB (N), nuclear fraction of nuclear factor-κB; NF-κB (C), cytosolic fraction of nuclear factor-κB.