Table 2.

Summary of hNek6 variants thermal shift inhibitor screen. A thermal shift of at least 2 °C (ΔT_m ≥ 2 °C) is depicted in italics and a significant thermal shift (ΔT_m > 4 °C) is depicted in bold. (D: dephosphorylated).

CompoundDescription	Nek6wt	Nek6wtD	Nek6(S206A)	Nek6(S206A)D	Nek6(Δ1-44)
Alsterpaullone	0.0	0.1	1.4	2.9	−0.5
Alsterpaullone, 2-Cyanoethyl	0.0	−0.1	2.5	2.5	−0.2
Aminopurvalanol A	0.3	−1.8	0.8	_	−0.2
Aminopyrazole1	−0.4	0.1	1.0	2.4	0.0
AR-A014418	0.3	−0.5	1.4	2.6	−0.2
Indolinone1	_	−0.4	−0.2	2.1	−0.5
Indolocarbazole1	−0.3	−0.1	1.7	0.7	−1.3
Isogranulatimide	1.2	0.6	6.5	2.3	−0.7
JNJ-7706621	0.0	0.6	0.7	2.1	−0.7
Pyrazolanthrone	0.3	−1.1	1.8	2.3	0.1
Staurosporine	0.3	−0.7	2.1	2.3	_
TPCA-1	0.0	−0.7	0.9	2.0	−1.7