Table 1.

Design and properties of siRNA targeting Renilla luciferase gene.

	Antisense ..ZW..	Sense ..XY..	IC50 (pM) ± SD	Tm (°C) ± SD	ΔTm (wt)	ΔTm (Parent)
wt	..U$\text{U}$..	..A$\text{A}$..	9.6 ± 0.5	67.8 ± 0.3	--	--
wtU9	..U$\text{U}$..	..A$\text{U}$..	15.4 ± 0.7	60.4 ± 0.2	7.6	7.6
wtC9	..U$\text{U}$..	..A$\text{C}$..	30.7 ± 0.6	59.5 ± 0.4	8.5	8.5
wtG9	..U$\text{U}$..	..A$\text{G}$..	15.8 ± 0.2	65.9 ± 0.2	2.1	2.1
wtU10	..$\text{U}$U..	..$\text{U}$A..	No active	58.2 ± 0.4	9.6	9.6
wtC10	..$\text{U}$U..	..$\text{C}$A..	No active	60.0 ± 0.1	7.8	7.8
wtG10	..$\text{U}$U..	..$\text{G}$A..	101 ± 0.7	64.5 ± 0.6	3.5	3.5
T10A10	..$\text{T}$$\text{L}$U..	..$\text{A}$A..	111 ± 0.8	58.3 ± 0.3	9.5	--
T11A9	..U$\text{T}$$\text{L}$..	..A$\text{A}$..	20.2 ± 0.6	59.1 ± 0.3	8.7	--
T10U10	..$\text{T}$$\text{L}$U..	..$\text{U}$A..	216 ± 0.6	55.2 ± 0.1	12.6	3.1
T10C10	..$\text{T}$$\text{L}$U..	..$\text{C}$A..	277 ± 0.9	54.1 ± 0.4	13.7	4.2
T10G10	..$\text{T}$$\text{L}$U..	..$\text{G}$A..	110 ± 0.9	55.7 ± 0.3	10.8	1.3
T11U9	..U$\text{T}$$\text{L}$..	..A$\text{U}$..	35.6 ± 0.4	56.4 ± 0.2	11.4	2.7
T11C9	..U$\text{T}$$\text{L}$..	..A$\text{C}$..	57.3 ± 0.5	55.6 ± 0.2	12.2	3.5
T11G9	..U$\text{T}$$\text{L}$..	..A$\text{G}$..	26.5 ± 0.8	58.4 ± 0.5	9.4	0.7