Figure 6.

TBBX induced Hsp90 hyper-acetylation and disrupted cyclin D1/Hsp90 and CDK4/Hsp90 interaction and leading to cyclin D1 and CDK4 degradation in H1299 lung cancer cells. (A) H1299 cells were incubated with or without 10 μM MG132 for 30 min before 10 μM TBBX stimulation for 24 h. Cells were harvested to detect cyclin D1 and CDK4 expression by Western blotting analyses. (B) H1299 cells were incubated with 10 μM TBBX for 24 h. Cells were then harvested and immuno-precipitation analysis was down with anti-Hsp90 antibody. The immuno-precipitates were then detected by Western blotting with anti-cyclin D1, anti-CDK4 and anti-acetyl-lysine antibodies as described in Materials and Methods. Data shown are representative of at least three independent experiments. Significant difference was observed from the control group (* p < 0.05).