Table 1.
KD values of the lignans in different volume ratios of the n-hexane-ethyl acetate-methanol-water solvent system for HSCCC separation (compound 1, justicidin B; compound 2, justicidin A; compound 3, 6'-hydroxy justicidin C; compound 4, lignan J1).
| n-Hexane–Ethyl Acetate–Methanol–Water | KD | ||||||
|---|---|---|---|---|---|---|---|
| Compound 1 | Compound 2 | Compound 3 | Compound 4 | ||||
| 1:1:1:1 | >20 | >20 | >20 | >20 | |||
| 1.2:1:1.2:1 | 1.16 | 1.89 | 4.43 | 13.04 | |||
| 1.3:1:1.3:1 | 0.93 | 1.48 | 3.48 | 9.79 | |||
| 1.4:1:1.4:1 | 0.62 | 1.02 | 2.35 | 7.17 | |||
| 1.5:1:1.5:1 | 0.48 | 0.76 | 1.92 | 4.58 | |||
| 1.8:1:1.8:1 | 0.19 | 0.30 | 0.79 | 2.34 | |||
| 2:1:2:1 | 0.12 | 0.20 | 0.54 | 1.49 | |||
| 2.5:1:2.5:1 | 0.07 | 0.16 | 0.31 | 0.88 | |||
| 3:1:3:1 | 0.06 | 0.10 | 0.28 | 0.80 | |||
| 4:1:4:1 | 0.03 | 0.04 | 0.12 | 0.32 | |||