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. 2015 Feb 25;20(3):3758–3775. doi: 10.3390/molecules20033758

Figure 3.

Figure 3

Effect of tyrosol on caspase-3 activity in H9c2 cardiomyocytes during I/R. Cells were pretreated with tyrosol at 0.10, 0.25, or 0.50 mM for 24 h, then subjected to I/R in the presence of the same levels of tyrosol. Caspase-3 activity was assessed using SensoLyte™ (Fremont, CA, USA) Homogeneous Rh110 Caspase-3 Assay Kits. Fluorescence ratios (Ex/Em = 485 nm/538 nm) are shown. Results are expressed as -fold that of control, and means ± SEs (n = 3) are shown. a: p < 0.05 compared with control, b: p < 0.05 compared with I/R cells, c: p < 0.05 compared with I/R + Tyrosol 0.10 mM cells.