Figure 7.

Detection by fluorescent microscope of acridine orange/ethidium bromide double stained DU 145 cells treated with MS17 for 12, 24, 48 and 72 h. Untreated viable cells are uniformly pale-green (white arrow); Early apoptotic cells shows characteristic loss of membrane integrity and chromatin condensation, stained bright-green to yellow (red arrow); Late apoptotic cells stained yellow-orange or red colour, with a condensed or fragmented chromatin (yellow arrow); Necrotic cells showed bright orange-red in appearance (blue arrow). Magnification 100×.