Table 3.
Strain and plasmid list.
| Strain or Plasmid | Relevant Genotype | Source or Reference |
|---|---|---|
| Strains | ||
| S. aureus | ||
| Newman D2C | Wild-type SrtA positive; nonhemolytic; coagulase Negative | ATCC25904 |
| ΔSrtA | srtA::Emr; isogenic mutant of Newman D2C | |
| E. coli | ||
| BL21 | Expression strain, F− ompT hsdS(rB− mB−) gal dcm (DE3) | Invitrogen |
| Plasmids | ||
| pGEX-6P-1 | Expression vector | Amersham |
| pGSrtAΔN59 | pGEX-6P-1 with srtA gene | This study |
| G167A | pGSrtAΔN59 derivative, for the substitution of Gly167 with alanine | This study |
| V193A | pGSrtAΔN59 derivative, for the substitution of Val192 with alanine | This study |