Figure 5.

P. chinense extract attenuated t-BHP-induced apoptosis by inhibiting mitochondrial apoptotic pathway in L02 cells. (A) L02 cells were pretreated with different concentrations of P. chinense extract for 12 h, followed by exposition to t-BHP for 30 min, and then incubated with JC-1 dye for another 30 min. The cell MMP was measured using In Cell Analyzer 2000. (B) L02 cells were treated as described above and cell nuclei were stained with Hoechst 33342 and imaged using an In Cell Analyzer 2000. (C) L02 cells were treated as described above and were harvested for western blotting analysis using indicated antibodies. Similar results were obtained from two or three independent experiments. Magnification 200×.