Figure 3.

BAT3 inhibits NF-κB-driven reporter gene expression. L929sA cells stably transfected with p(IL6κB)₃50hu.IL6P-Luc and PGK-Gal were left untreated (untr) or were treated with 2000 IU/mL TNF for 4 h either alone or following a 24 h pretreatment with different doses of BAT3. Lysates were prepared for quantification of luciferase reporter gene expression levels and correction for potential cytotoxicity by evaluating corresponding constitutively expressed galactosidase levels. The relative induction factor is defined as the amount of luciferase produced in TNF-treated cells after normalization for galactosidase expression compared with the non-induced state. Statistical significant repression by BAT3 is indicated as ** p < 0.01 or *** p < 0.001.