Table 5.
Primer | Forward Primer Sequence | Reverse Primer Sequence | Temperature (°C) | Size of Amplified Fragment (bp) |
---|---|---|---|---|
First primer pairs | 5' TCTTCGCCAAAAGCACTCTGA 3' | 5' TGGTGATTTCAGCGTACCGAATTA 3' | 60 | 858 |
Nested primer pairs | 5' CCTCTTTCGAAAGAAGTCGGGG 3' | 5' ACAATTTGGACTTTCCGCCCT 3' | 57.4 | 700 |