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. 2015 Feb 23;20(3):3647–3666. doi: 10.3390/molecules20033647

Table 5.

Primers designed for nested PCR analysis of genomic DNA of putative transformants.

Primer Forward Primer Sequence Reverse Primer Sequence Temperature (°C) Size of Amplified Fragment (bp)
First primer pairs 5' TCTTCGCCAAAAGCACTCTGA 3' 5' TGGTGATTTCAGCGTACCGAATTA 3' 60 858
Nested primer pairs 5' CCTCTTTCGAAAGAAGTCGGGG 3' 5' ACAATTTGGACTTTCCGCCCT 3' 57.4 700