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Synthesis of new phospholipid-protein conjugates by amide coupling and click chemistry methods. Reagents and conditions: (i) 0.1 M bicarbonate buffer–DMSO 9:1, +4 °C, 12 h; (ii) PE azide, CuSO4:TBTA 1:1.1, ascorbic acid, 1× PBS-DMSO–t-BuOH 3:2:0.1, v/v/v; (iii) KMnO4, NaIO4, t-BuOH–H2O 9:1, v/v, rt, 12 h; (iv) succinimide ester, N,N′-diisopropylcarbodiimide, DMSO, rt, 12 h; (v) proteins 2,3,8, 0.1 M bicarbonate buffer–DMSO 9:1, v/v, rt, 12 h.
