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. 2015 Apr 15;20(4):6794–6807. doi: 10.3390/molecules20046794

Figure 2.

Figure 2

Tan-IIA1, Sal-A and Sal-B inhibited the ANE-induced collagen accumulation and transcription of procollagen genes COL1A1 and COL3A1. (A) Tan-IIA1, Sal-A and Sal-B inhibited the ANE-induced collagen accumulation; n = 5, * p < 0.05 and ** p < 0.01 vs. the group only treated with ANE. (B) Tan-IIA1, Sal-A and Sal-B decreased the promoter activity of human procollagen genes COL1A1 and COL3A1. (a) Double-enzyme digestion of pCol1A1-p-Luc and pCol3A1-p-Luc plasmids; (b) luciferase reporter plasmid assay; the results are shown as the fold change of the 10% FCS control. n = 5, * p < 0.05 and ** p < 0.01 vs. the control containing 10% FCS and ANE (12.5 μg/mL). (C) qRT-PCR assay. MOMFs were treated as in the gelatin zymography assay. The 2−ΔΔCt values were calculated, and the results are expressed as the fold change of the 10% FCS control. n = 5, * p < 0.05 and ** p< 0.01 vs. the control containing 10% FCS and ANE (12.5 μg/mL).