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. 2015 Apr 15;20(4):6794–6807. doi: 10.3390/molecules20046794

Figure 3.

Figure 3

Effect of Tan-IIA1, Sal-A and Sal-B on MMP activity and TIMP expression after ANE treatment. (A) Effect of Tan-IIA1, Sal-A and Sal-B on MMP-2/-9 activity (left: the graph of the gelatin zymography assay; right: the statistical results). MOMFs were cultured in 10% FCS DMEM medium with or without ANE (12.5 μg/mL) and simultaneously treated with or without Tan-IIA1 (6.25 μM), Sal-A (3.125 μM) and Sal-B (12.5 μM). Lane 1 (ANE-only treated control): FCS + ANE; Lane 2 (blank control): FCS; Lane 3 (Tan-IIA1-treated group): FCS + ANE + Tan-IIA1; Lane 4 (Sal-A-treated group): FCS + ANE + Sal-A; Lane 5 (Sal-B-treated group): FCS + ANE + Sal-B. n = 3, ** p < 0.01 vs. the ANE only-treated control. (B) Effect of Tan-IIA1, Sal-A and Sal-B on the expression of MMP-1, MMP-2, MMP-9, MMP-13, TIMP-1 and TIMP-2 by qRT-PCR assay; the result was shown as the fold change of the 10% FCS group. n = 5, * p < 0.05 and ** p < 0.01 vs. the FCS + ANE group.