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. 2016 Nov 9;21(11):1490. doi: 10.3390/molecules21111490

Figure 4.

Figure 4

Effect of SDS on LPS-induced IκB-α degradation and NF-κB activation in RAW 264.7 cells. RAW 264.7 cells were pretreated with various concentrations of SDS for 1 h, followed by stimulated with LPS (1 μg/mL) for 30 min. The cells were harvested, and then protein was prepared for the detection of NF-κB p65 from in cytosol (Cy) or nuclear (Nu). α-tubulin (Cy) and Topo I (Nu) are loading controls of corresponding cellular fractions. Data are expressed as mean ± SD of three independent experiments. * p < 0.05, *** p < 0.001, versus LPS alone.