Figure 6.

Activation of p53 mediated transcriptional activity by γ-bisabolene. Western blot analysis of lysates from treated and mock cells was performed; the blots were probed with primary and secondary antibodies (A); Relative band intensity of phospho-p53 or phosphor-p21 was normalized by β actin, compared to the mock cells, and quantified using imageJ based on triplicate replicates of each experiment (B); Relative fold levels in treated and mock cells appear as ratio of indicated mRNA/GAPDH mRNA after performing real time PCR assays (C). **, p value < 0.01; ***, p value < 0.001 compared with untreated cells.