Figure 4.

The effect of FAC overload on expression levels of iron homeostasis related gene. (A) FAC overload induced upregulation of iron homeostasis related protein expression. Expression of β-actin was used as internal control. Results are shown as mean ± SEM of three independent experiments. Statistically significant differences are labeled as * p < 0.05 and ** p < 0.01, compared with the control groups, using unpaired Student’s t test; (B) Schematic representation of the activation of Hepcidin regulation-associated signal transduction pathway in HH4 cells with FAC treatment.