Table 3.

The ¹H-NMR, ¹³C-NMR chemical shift change of the baicalin–berberine complex, contrasted with baicalin.

Baicalin (δ ppm)	Baicalin–Berberine Complex (δ ppm)	Difference (ppm)
2’’, 3’’, 4’’-OH, 5.52–5.31	5.04–4.92	0.39–0.48
1’’-H, 5.26	4.96	0.30
5’’-H, 4.08	3.58	0.50
2’’, 3’’, 4’’-H, 3.47–3.42	3.32–3.29	0.13–0.15
6”-C, 170.1	171.3	1.2
1”-C, 99.9	100.6	0.7
5”-C, 75.5	76.0	0.5
3”-C, 75.2	73.8	1.4
4”-C, 71.3	72.2	0.9