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. 2016 Jun 22;21(6):818. doi: 10.3390/molecules21060818

Figure 1.

Figure 1

Effects of MHE on (a) cell viability and (b–e) the production of inflammatory mediators in RAW 264.7 cells. Cells were pretreated with MHE for 1 h, followed by a 24 h incubation with LPS. (a) Cell viability was measured using a CCK assay; (b) NO content in conditioned medium was determined using Griess reagent; (c–e) Cytokine levels in the medium were measured using ELISA. Con: control; Dex: 10 μM dexamethasone. As a control, cells were incubated with the vehicle alone. * p < 0.01 and ** p < 0.001 were calculated from comparing with LPS-stimulation value.