Figure 10.

Effect of test compounds on H₂O₂-induced late apoptotic/necrotic cell death of WI-38 cells. (A) Untreated control; (B) Cells treated only with H₂O₂; (C) Stearic acid ester of Q3G; (D) Oleic acid ester of Q3G; (E) Linoleic acid ester of Q3G; (F) ALA ester of Q3G; (G) EPA ester of Q3G; (H) DHA ester of Q3G; (I) Q3G; (J) Quercetin; (K) Number of late apoptotic/necrotic cells in relation to the treatments. Cells were pre-incubated with test compounds for 48 h and then oxidative injured by 3 h incubation with H₂O₂. Cells were rinsed with PBS, stained with necrosis detection reagent and observed under a fluorescence microscope.