Figure 4.

Effects of SPN on the protein expressions of HIF-1α and PHD-2 in ARPE-19 cells at 24 h of normoxia or hypoxic conditions. (a): HIF-1α protein expression; (b): PHD-2 protein expression. The results are representative of at least three independent experiments run in triplicate and expressed as the mean ± SD (n = 9). ** p < 0.01 vs. vehicle plus CoCl₂-treated group. The control cells were treated with vehicle (culture medium containing 0.1% DMSO). Beta-actin was used as the loading control.