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. 2016 Oct 10;21(10):1334. doi: 10.3390/molecules21101334

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) H1299 and A549 cells were immunostained by the anti-CD71 antibody which binds to transferrin receptors (TfR) and by the isotype antibody which served as a control of non-specific binding. Median fluorescence intensity (MFIs) were quantified via flow cytometry; (b) H1299 and H460 cells were immunostained by the anti- CD71 antibody and by the isotype antibody (Data points indicate mean ± SD, n = 2. *** p < 0.001); (c) The cellular uptake of bPEI and HAI-SPDP-bPEI polyplexes was determined in TfR overexpressing cells H1299 and TfR low expressing cells A549. Polyplexes were prepared with 50 pmol of siRNA fluorescently labeled with Alexa Fluor 488 at N/P = 5 and transfected for 24 h. The MFIs were quantified by flow cytometry. (Data points indicate mean ± SD, n = 2. ** p < 0.01, *** p < 0.001); (d) The cellular uptake of bPEI and HAI-SPDP-bPEI polyplexes was determined in TfR overexpressing cells H1299 and TfR low expressing cells H460. (Data points indicate mean ± SD, n = 3. *** p < 0.001).