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. 2016 Mar 9;21(3):315. doi: 10.3390/molecules21030315

Table 1.

The influence of the volume ratios of mobile phase on the separation effect of crude extracts of flower buds of P. ginseng (CEFBPG).

Test No. Volume Ratios of Moble Phase (Acetonitrile–Water) (n = 3) Peak Purity of Ginsenoside F5 (%) a,b RSD (%) (Ginsenoside F5) Peak Purity of Ginsenoside F3 (%) a,b RSD (%) (Ginsenoside F3)
1 28:72 96.55 ± 1.18 1.22 94.69 ± 1.25 1.32
2 32:68 96.19 ± 1.35 1.41 90.47 ± 2.45 2.71
3 32:68 → 28:72 (15 min) 95.87 ± 0.87 0.91 94.09 ± 1.02 1.08
4 32:68 → 28:72 (10 min) 96.96 ± 1.04 1.08 97.18 ± 1.27 1.30

a Peak purity: calculated by the peak area normalization method; b Results of peak purity are the means ± SD of four independent experiments performed in triplicate.