Table 1.
Test No. | Volume Ratios of Moble Phase (Acetonitrile–Water) (n = 3) | Peak Purity of Ginsenoside F5 (%) a,b | RSD (%) (Ginsenoside F5) | Peak Purity of Ginsenoside F3 (%) a,b | RSD (%) (Ginsenoside F3) |
---|---|---|---|---|---|
1 | 28:72 | 96.55 ± 1.18 | 1.22 | 94.69 ± 1.25 | 1.32 |
2 | 32:68 | 96.19 ± 1.35 | 1.41 | 90.47 ± 2.45 | 2.71 |
3 | 32:68 → 28:72 (15 min) | 95.87 ± 0.87 | 0.91 | 94.09 ± 1.02 | 1.08 |
4 | 32:68 → 28:72 (10 min) | 96.96 ± 1.04 | 1.08 | 97.18 ± 1.27 | 1.30 |
a Peak purity: calculated by the peak area normalization method; b Results of peak purity are the means ± SD of four independent experiments performed in triplicate.