Figure 3.

(a) Scratch wound assay. Wounds were generated after cell confluence. Cell migration and wound closure were assessed after 72 h, 4× images of primary culture (control sample and treated sample) at baseline and after 72 h, scale bar 1000 µm; (b) Areas of migration measured in the control sample and treated sample on days 0 and 3; (c) Morphological changes in the primary culture after eribulin treatment, 4× images, scale bar 1000 µm. After 72 h of treatment, eribulin induced a decrease in cell size, the cells lost some of the cytoplasmic architecture features, rounded up and were reduced in number; (d) Western blot analysis of apoptosis (p-53, Bax, Casp3 and Casp9) and migration-related proteins (Rho) and fold change in protein expression.