Figure 4.
Effect of agrimoniin-enriched fraction PE4 on UVB-induced COX-2 expression. HaCaT cells were irradiated with 30 mJ/cm2 UVB and treated with 25 µg/mL PE4. Non-irradiated, untreated cells served as control. Subsequently, the cells were centrifuged on slides and COX-2 expression was measured. The graph shows the percentage of COX-2 positive cells per 100 calculated cells in two staining approaches. The bar corresponds to 50 µm. Data are expressed as mean ± SD and the statistical significance was determined compared to the irradiated, untreated control (** p < 0.01).