Table 1.

Quenching reactive constants of HSA by SSC.

Fluorescence	T (°C)	Detection	Kq (L·mol−1·S−1)	R
Conventional	26	λex = 280 nm	4.19 × 1012	0.996
	36	λex = 280 nm	1.17 × 1012 *	0.965
Synchronous	26	∆λ = 15 nm	2.97 × 1011	0.963
	26	∆λ = 60 nm	2.89 × 1012	0.995

R, linear correlation coefficient; * p < 0.05 compared with conventional fluorescence at 26 °C by the paired t-test.